High-Temperature Crystallization of Thermostable T1 Lipase
نویسندگان
چکیده
منابع مشابه
Crystallographic Analysis of Ground and Space Thermostable T1 Lipase Crystal Obtained via Counter Diffusion Method Approach
Three-dimensional structure of thermostable lipase is much sought after nowadays as it is important for industrial application mainly found in the food, detergent, and pharmaceutical sectors. Crystallization utilizing the counter diffusion method in space was performed with the aim to obtain high resolution diffracting crystals with better internal order to improve the accuracy of the structure...
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BACKGROUND Pichia guilliermondii was found capable of expressing the recombinant thermostable lipase without methanol under the control of methanol dependent alcohol oxidase 1 promoter (AOXp 1). In this study, statistical approaches were employed for the screening and optimisation of physical conditions for T1 lipase production in P. guilliermondii. RESULT The screening of six physical condit...
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Less sedimentation and convection in a microgravity environment has become a well-suited condition for growing high quality protein crystals. Thermostable T1 lipase derived from bacterium Geobacilluszalihae has been crystallized using the counter diffusion method under space and earth conditions. Preliminary study using YASARA molecular modeling structure program for both structures showed diff...
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Theromostable lipases have wide range of biotechnological applications in the industry. Therefore, there is always high interest in investigating their features and operating conditions. However, Lipase production is a challenging and complex process due to its nature which is highly dependent on the conditions of the process such as temperature, initial pH, incubation period, time, inoculum si...
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ژورنال
عنوان ژورنال: Crystal Growth & Design
سال: 2007
ISSN: 1528-7483,1528-7505
DOI: 10.1021/cg050506z